前言
Experiment 1 Preparation of Plasmid DNA
Experiment 2 Restriction Enzyme Digestion and Agarose Gel Electrophoresis
Experiment 3 Polymerase Chain Reaction (PCR)
Experiment 4 DNA Purification and Construction of Recombinant DNA
Experiment 5 Preparation of Competent E. coli Cells
Experiment 6 Transformation of Recombinant DNA
Experiment 7 Identification and Characterization of Positive Clones
Experiment 8 Isolation of Total RNA from Animal Tissues
Experiment 9 Molecular Hybridization
Experiment 10 Reverse Transcription – Polymerase Chain Reaction (RT-PCR) and TA-cloning
Experiment 11 Expression and Purification of a Fusion Protein from E. coli
Experiment 12 Electrophoretic Mobility Gel Shift Assay (EMSA) to Detect DNA-Protein Interactions
Appendix I Brief Introduction to the NCBI Database
Appendix II Vector Information
Appendix III Instructions on How to Use the Equipment
References
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