Chapter 1 Introduction to theM edicalBiology Laboratory
1.1 Objectives of a Teaching Medical Biology Laboratory
1.2 Laboratory Rules
1.3 Laboratory Safety
1.4 Common Laboratory Equipments
1.5 Experimental Methods in Medical Biology
1.6 Evaluation of Experimental Data and Laboratory Reports
Chapter 2 Experiments in M edicalBiology
Experiment 1 The Use of Glass Pipets and Pipettors
Experiment 2 Amphoteric Ionization and Isoelectric Point Determination of Proteins
Experiment 3 Quantitative Determination of Protein Concentrations by Coomassie Brilliant Blue Assay
Experiment 4 Quantitative Determination of Proteins by Folin‐phenol Assay
Experiment 5 Determination of Proteins by UV Absorption
Experiment 6 Separation of Amino Acid Mixtures by Ion‐exchange Chromatography
Experiment 7 Separation of Serum γ‐globulins by Ion‐exchange Chromatography
Experiment 8 Separation of Serum Proteins by Cellulose Acetate Membrane Electrophoresis
Experiment 9 Separation and Purity Determination of Serum γ‐globulins by SDS‐polyacrylamide Gel Electrophoresis
Experiment 10 Determination of Serum Glutamic‐pyruvic Transaminase Activity
Experiment 11 Determination of Serum Urea Nitrogen Content
Experiment 12 Determination of Blood Glucose Concentration
Experiment 13 Subculture of Adhesion Cells
Experiment 14 Counting and Determination of Live／Dead Cells
Experiment 15 Preparation of Cell Samples for Observation of Cell Morphologies
Experiment 16 Mitosis and Meiosis of Cells
Experiment 17 Intracellular Distribution of Biomacromolecules
Experiment 18 Isolation of Subcellular Components
Experiment 19 Preparation of Chromosome Samples from Mouse Bone Marrow Cells
Experiment 20 Banding Pattern of Human Chromosomes
Experiment 21 Colony Forming Efficiency and Total Protein Analysis of Tumor Cells
Experiment 22 Isolation ,Amplification and Identification of the β‐actin Gene Fragment from Mouse Liver Cells
Experiment 23 Purification of Target DNA and Generation of Recombinant DNA
Experiment 24 Transformation and Screening of Recombinant DNA
Experiment 25 Determination of Serum Proteins Using Western Blotting
